《植物生理学报》 2016, 52(10): 1519-1526

刨花润楠组织培养技术的初步研究

陈怡佳, 鲁好君, 赵帅, 湛欣, 邓小梅^*

华南农业大学林学与风景园林学院, 广东省森林植物种质创新与应用重点实验室, 广州510642

通信作者：邓小梅；E-mail: dxmei2006@scau.edu.cn

摘　要：

以刨花润楠种子为外植体, 采用丛芽发生途径, 初步建立其组培快繁技术体系。结果表明, GA₃浸泡种子可显著提高发芽率, 缩短发芽时间。子叶节最易诱导不定芽, 诱导芽数最多。刨花润楠丛芽再生能力较弱, 培养过程中愈伤组织多, 整体情况较差。较为适宜的增殖方式为外植体在MS+6-BA 3.0 mg•L^-1+IBA 0.01 mg•L^-1+AgNO₃ 1.0 mg•L^-1上预培养10 d, 再转入MS+6-BA 3.0 mg•L^-1+IBA 0.01 mg•L^-1中培养, 增殖系数可达3.52。生根培养基为1/2MS+IBA 0.2 mg•L^-1, 生根率可达91%。

关键词：刨花润楠; 组织培养; 基本培养基; AgNO₃

收稿：2016-05-16   修定：2016-09-19

资助：国家“十二五”科技支撑项目(2012BAD01B04)和广东省林业科技创新项目(2011KJCX002)。

Primary study of tissue culture of Machilus pauhoi

CHEN Yi-Jia, LU Hao-Jun, ZHAO Shuai, ZHAN Xin, DENG Xiao-Mei^*

College of Forestry and Landscape Architecture, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agriculture University, Guangzhou 510642, China

Corresponding author: DENG Xiao-Mei; E-mail: dxmei2006@scau.edu.cn

Abstract:

Tissue culture of Machilus pauhoi were studied through clustered bud cultivation from seeds as explants. The results showed that the germination rate of Machilus pauhoi seeds was effectively improved by soaking the seeds with gibberellin. The cotyledon node was the best part for induction. The best way to proliferation was the plantlets pre-culture on the medium with AgNO₃ (MS+6-BA 3.0 mg•L^-1+IBA 0.01 mg•L^-1+AgNO₃ 1.0 mg•L^-1) for ten days, then transplanted into the same medium without AgNO₃ (MS+6-BA 3.0 mg•L^-1+IBA 0.01 mg•L^-1), the proliferation coefficient was up to 3.52. The better rooting medium was 1/2MS+IBA 0.2 mg•L^-1, the rooting rate was above 91%.

Key words: Machilus pauhoi; tissue culture; basal medium; AgNO₃